Location: Plant Genetic Resources
Project Number: 8060-21000-025-01
Start Date: Dec 01, 2011
End Date: Nov 30, 2016
The research will focus on the 2,500 accessions that comprise the USDA-ARS Malus collection maintained in Geneva, NY. Funds permitting, genomic analysis of additional apple varieties and collections from non-ARS sources may also be included. Genotyping-by-sequencing (GBS) techniques will be used to discover SNPs preserved genome-wide within Malus sp. accessions. DNA isolation and preparation will be done by ARS, but GBS library construction and sequencing will be performed at Cornell University core facility. GBS data will be processed and analyzed to address questions about the diversity of the USDA-ARS Malus collection, including diversity and differentiation, domestication and introgression history, signatures of selections and cultivar pedigree estimation. In addition, these data will serves as the basis for developing markers for use in breeding, and collection management. Discovered SNPs and genetic characterization of the apple collection will be made publicly available.