Location: Cool and Cold Water Aquaculture Research
Project Number: 8082-32000-005-05
Start Date: Jan 15, 2012
End Date: Jan 14, 2016
The relative frequency of resting B, plasmablasts, plasma cells, proliferating T cells, and activated macrophages will be determined in the three trout lines using flow cytometry. Fluorescently labeled antibodies specific to trout IgM, IgD, Pax-5, the proliferation marker Edu, CD3, TNF-alpha, and IL1-beta will be used. Tissue from four immune sites, peripheral blood, spleen, anterior kidney, and posterior kidney will be cultured in vitro with one of two mitogens, either E.coli lipopolyssacharide (LPS) or Flavobacterium LPS, and cells analyzed at selected time points after 0-7 days of induction. Undergraduate students from William and Mary, Department of Biology will work on this project during the academic year and during summer internships.