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United States Department of Agriculture

Agricultural Research Service

Research Project: Breeding Cool Season Food Legumes for the Pacific Northwest and Northern Plains States of the Usa

Location: Grain Legume Genetics Physiology Research

Project Number: 5348-21000-029-09
Project Type: Specific Cooperative Agreement

Start Date: Sep 20, 2012
End Date: Sep 30, 2014

Objective:
The objectives of this study are to 1) Develop improved varieties of spring and autumn sown peas and lentils with superior yield, plant architecture, food quality and processing characteristics; 2) Screen breeding lines for resistance to soilborne pathogens (Aphanomyces euteiches, Pythium spp., Fusarium oxysporum, Fusarium solani and Rhizoctonia spp.); foliar fungal pathogens (powdery mildew) and viral diseases (Bean Yellow Mosaic Virus Pea Enation Mosaic Virus and Pea Seed-borne Mosaic Virus) under greenhouse and/or field conditions; 3) Establish observation nurseries, preliminary and advanced yield trials in WA and ID; 4) Evaluate selected breeding lines for end product utilization characteristics.

Approach:
Crosses of spring- and autumn-sown legumes will be made in the greenhouse during the Autumn and Winter Cycles and in the field during the summer. Parental lines will be selected based on performance in yield trials, in screening nurseries and on pedigree. The primary objectives will be tolerance to soilborne pathogens, resistance to foliar fungal pathogens, resistance to viruses, increased yield, improved agronomic characteristics (i.e. standability, maturity, plant architecture, reduced pod shatter and pod drop) and improved quality characteristics (seed shape, size and color, cooking time, etc). Segregating families and breeding lines will be screened for tolerance to Aphanomcyes root rot, Fusarium root rot, Fusarium wilt (Races 1 and 2) and other soilborne diseases in root rot disease nurseries. Plant materials will be scored at full bloom and flat pod stages for disease reaction using a rating scale of 1(no symptoms) to 5 (dead plant). Segregating families and breeding lines will also be tested for resistance to PEMV and PSbMV at Oregon State University. Breeding lines with desirable disease resistance and other agronomic traits will be selected. Advanced yield trials will be conducted at four locations in Washington and Idaho to identify high yielding breeding lines with desirable agronomic traits including standability, early maturity, seed color and seed size and shape. Up to 20 advanced breeding lines and two to three appropriate checks in each market class will be sown in replicated plots at each location. Approximately 25 additional advanced breeding lines of each market class will be evaluated for the first time in replicated plots in a preliminary yield trial conducted at the Spillman Farm. A single plot each of approximately 500 breeding lines of each species will also be evaluated in a preliminary screening nursery at Spillman Farm. Based on the results of yield trials, disease screening and quality evaluations, selections that have superior performance compared to standard varieties will be considered for release. Advanced breeding lines will be evaluated for color and cooking qualities using protocols developed at ICARDA. Seed weight before and after soaking, percentage water uptake, cooking times, and appearance before and after cooking will be determined for all advanced breeding lines.

Last Modified: 7/27/2014
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