Location: Arthropod-Borne Animal Diseases Research
Project Number: 5430-32000-005-17
Start Date: Feb 01, 2012
End Date: Sep 30, 2014
The National Veterinary Stockpile Recommendations list Rift Valley Fever (RVF) as the third most important agricultural threat to U.S. livestock. There are new diagnostic assays available that have not been evaluated as to their effective timeframe for use post-infection and sensitivity. The main objectives of this proposal are: 1. To determine the optimal time points post infection and diagnostic samples (blood, saliva etc.) for detection of RVFV with these assays, and 2.) To evaluate the compatibility of these assays with a DIVA control strategy which will be determined by comparing the diagnostic results of vaccinated sheep with wild-type infected sheep. These studies may include evaluating the ability of diagnostic assays to detect infectious virus, viral antigen(s) and virus-specific nucleic acids in insects.