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United States Department of Agriculture

Agricultural Research Service

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Research Project: Assessment of Ankara Virus As a Vector for African Swine Fever Virus and Classical Swine Fever Virus Proteins

Location: Foreign Animal Disease Research

Project Number: 1940-32000-056-14
Project Type: Specific Cooperative Agreement

Start Date: Jul 01, 2012
End Date: Dec 31, 2014

Objective:
This collaborative research agreement seeks to explore the efficacy of Ankara Virus (APV) as a vector for expressing African Swine Fever Virus (ASFV) and Classical Swine Fever Virus (CSFV) proteins. The specific objectives of this collaborative research project are: 1. Construct APV vectors expressing specific ASFV/CSFV genes. 2. Evaluate the protective effects of the mutated APV vectors.

Approach:
1. USDA-ARS will identify and provide selected recombinant African Swine Fever Virus (ASFV) and Classical Swine Fever Virus (CSFV) genes which have known immunogenetic properties to the Univeristy of Wisconsin (UW). UW will manipulate the Ankara Virus (APV), introducing the recombinant ASFV/ CSFV genes, resulting in a mutated virus which, when replicated will express proteins encoded by the ASFV/ CSFV genes. 2. Resulting mutated viruses will be tested in swine in vivo by USDA-ARS. Challenge studies against and the presence of ASFV/CSFV-related signed and mortality rates will be assessed. Upon successful trails utilizing homologous virus, further studies will be conducted utilizing additional strains.

Last Modified: 9/23/2014
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