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United States Department of Agriculture

Agricultural Research Service

Research Project: Translational Genomics for Recessive Disease Resistance in Cucumber (Ncsu)

Location: Vegetable Crops Research Unit

2012 Annual Report


1a.Objectives (from AD-416):
a. Allelism tests of downy mildew resistance loci in cucumber. b. Molecular mapping of downy mildew resistance loci. c. Conduct an outreach program.


1b.Approach (from AD-416):
a. Allelism tests of downy mildew resistance loci in cucumber: inbred lines carrying multi-source downy mildew resistance genes will be crossed and segregation of resistance will be evaluated in multi-year and location field and greenhouse trials.

b. Molecular mapping of downy mildew resistance: Multiple mapped populations will be developed and phenotypic data of downy mildew resistance will be collected. Genomic DNAs of segregation populations and parental lines will be used for molecular marker analysis to identify markers for downy mildew resistance major QTL.

c. The outreach program will enhance communication with various segments of the industry, and educate the public on the need for sound scientific practices to resolve biological problems. The outreach target groups will include the cucumber growers, the private seed companies, cucumber scientific community, the youth, and the general public.


3.Progress Report:

Development of mapping populations for quantitative trait loci (QTL) mapping of downy mildew resistance is under way. Parental lines and F2/F3 segregating populations have been planted in the field for phenotypic data collection.

This research relates to Objective 1, Determine the genetic basis of and initiate selection for carrot, onion, cucumber, and melon quality attributes influencing human nutrition and health, disease resistances, and yield and quality components, and stress tolerance in cucurbits, and perform field performance and quality trials; Objective 2, Utilize current biotechnology to discover and evaluate genetic variation and to map agriculturally important traits in Allium, Cucurbit, and Daucus germplasm, and to develop genetic and breeding stocks.


Last Modified: 12/19/2014
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