Location: Beneficial Insects Introduction Research
Project Number: 1926-22000-023-19
Start Date: Jul 01, 2012
End Date: Jun 15, 2015
We will use the tropical ash-based method to rear EAB larvae for tests with EAB larval parasitoids: Spathius sp (a newly imported gregarious, ectoparasitoid) and/or T. planipennisi (a gregarious endoparasitoids). Freshly cut tropical ash logs will be infested with fertilized EAB eggs laid on strips of un-bleached coffee filter paper. EAB egg-infested logs will be then placed into ventilated plastic boxes, and incubated under normal rearing conditions (27oC; RH 45 - 65%, and photoperiod L:D=16:8 hrs) prior to use in different tests for Objectives 1 and 2. A standardized size of the tropical ash logs (2.5 cm in diameter x 20 cm in length) will be used to rear EAB larvae for different tests. Optimal host larval stage, density, host to parasitoid ratio, temperature and photoperiod for the production of the fittest and most fecund parasitoids will be determined independently in a series of laboratory assays.