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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Accelerated Development of Commercial Hydrotreated Renewable Jet (Hrj) Fuel from Redesigned Oil Seed Feedstock Supply Chains

Location: Plant Physiology and Genetics Research

Project Number: 5347-21410-006-01
Project Type: Specific Cooperative Agreement

Start Date: Oct 01, 2012
End Date: Aug 31, 2016

Objective:
To 1) construct a high-density marker map for 800 diverse Brassica napus accessions from a world collection being grown by the University of Idaho and the USDA-ARS; 2) deliver processed genomic sequence to the USDA-ARS Soybase for further processing and public release; and 3) provide additional genomic sequencing as needed to validate marker/gene assignments.

Approach:
Leaf materials for DNA sequencing will be provided to Keygene company for DNA extraction, followed by genotyping-by-sequencing on the 800 accessions. Web based tools will be used to process sequence information, and deliver via internet to USDA-ARS Soybase for development of a public Brassica napus genomic sequence database. To assist genome wide association mapping strategies, Keygene will provide additional sequencing services as needed.

Last Modified: 8/30/2014
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