Project Number: 1235-51000-052-07
Project Type: Reimbursable

Start Date: Aug 01, 2012
End Date: Sep 30, 2016

Objective:
The objective of this agreement is to characterize the biological response to dietary exposure to naturally occurring bioactive food components. Specifically, the proposed studies will compare the availability and metabolism of these compounds from different preparations, and contrast the responses to these sources in terms of ability to reduce DNA damage and to monitor genetic, metabolomic, and proteomic expression changes resulting from chronic and acute exposures.

Approach:
The approach will be to conduct a series of phase 1 studies involving interventions with naturally occurring constituents of food products (in foods or isolated). Foods will be incorporated into controlled diets and administered to volunteers. The volunteers will consume the diets for a specified time, after which blood and urine samples will be collected. The blood and urine will be analyzed for a variety of biochemical markers related to cancer risk. Analyses will include appearance of nutrients in the blood/urine, markers of inflammation, DNA damage, gene expression, epigenetic makers, and metabolomics.