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United States Department of Agriculture

Agricultural Research Service

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Research Project: Identification of Gene Markers in Aflatoxin-Resistant Maize Lines Developed Through the Iita-Usda-Srrc Collaboration

Location: Food and Feed Safety Research

Project Number: 6054-42000-021-17
Project Type: Trust

Start Date: Jul 01, 2012
End Date: Jun 30, 2015

Objective:
1. Use the kernel screening assay (KSA) to screen seed of best segregating materials from International Institute of Tropical Agriculture (IITA) for aflatoxin-resistance, and to identify closely-related lines. 2. Conduct field trials of the six TZAR inbreds (as inbreds and hybrids) already developed and released by the IITA-Southern Regional Research Center (SRRC) collaborative project. 3. Conduct proteome analysis on the six TZAR inbred lines to identify resistance-associated proteins (RAPs)/potential markers.

Approach:
Use kernel screening assay (KSA) to screen International Institute of Tropical Agriculture (IITA) bred materials for aflatoxin accumulation to assist in their advancement through the collaborative breeding program. Conduct resistance field trials in southern U.S. to evaluate inbreds released through the program. Use 2 D gel electrophoresis to identify resistance-associated proteins (RAPs) in IITA-Southern Regional Research Center (SRRC) lines to discover potential gene markers. Once identified, characterize RAPs biochemically and physiologically using state-of-the-art techniques to further determine their potential as markers.

Last Modified: 10/25/2014
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