Project Number: 5310-21000-010-13
Start Date: Oct 01, 2013
End Date: Apr 30, 2015
Mikeal Roose and Chandrika Ramadugu (UCR) have expertise in conducting genetic analysis of individual clones by various methods including SSR analysis and sequencing of selected nuclear genes to determine if the individual trees are nucellar trees or hybrids. Genetic analysis of LAS populations will be conducted at USDA ARS Riverside in cooperation with Yongping Duan. Lee's lab (USDA ARS, Ft. Pierce) has been conducting HLB research for several years. Clonal propagations of selected trees will be maintained in Ed Stover's greenhouse (USDA ARS, Ft. Pierce). Insect transmissions will be conducted in cooperation with David Hall (USDA ARS, Ft. Pierce). Year 1: Collection of samples from individual trees and tissues for genetic analysis; establishment of promising clonal populations in greenhouse; propagation of trees for insect and graft transmission; start genetic analysis of host and pathogen Year 2: Establish insect and graft transmission studies and analyze plants over a period of two years at 3-month intervals; complete genetic analysis of host and pathogen from various tissues; analyze results and publish.