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United States Department of Agriculture

Agricultural Research Service

Research Project: Development of Diagnostic Screening Tools and Evaluation of Transgenic Plants for Cotton Leaf Curl Virus (Clcuv) Resistance

Location: Genomics and Bioinformatics Research Unit

Project Number: 6402-21310-004-17
Project Type: Specific Cooperative Agreement

Start Date: Sep 01, 2012
End Date: Sep 30, 2015

Objective:
The goal of the Cotton Productivity Enhancement Program is to identify sources of resistance to Cotton Leaf Curl Virus (CLCuV) and transfer them into adapted lines. This program will investigate various possibilities for control including transgenic methods to combat the virus. The program addresses the urgent need to identify alternate sources of resistance to CLCuV, currently a major disease of cotton in Pakistan, to increase U.S. preparedness should CLCuV become established in the U.S.

Approach:
To preserve the effectiveness of resistant cotton lines, it is essential to be able to identify, characterize and monitor the different strains of Cotton Leaf Curl Virus (CLCuV). New tools (predominately based on molecular biology approaches) will be needed to determine the range of different viral strains, track their changes and study how these changes affect virulence. Assessing the range of CLCuV strains, rates of change, and the effect on virulence will require the expertise of a virologist(s) well versed in the area of molecular biology. In addition, resistant transgenic plants developed using ribonucleic acid (RNAi) technologies need to be advanced multiple generations and tested for suitable expression and stability of the RNAi constructs. This will be done in the model tomato system before transferring into cotton.

Last Modified: 9/20/2014
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