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United States Department of Agriculture

Agricultural Research Service

Research Project: Production of Antibodies to Detect for Viruses from the Grapevine Leafroll Complex by Elisa

Location: Horticultural Crops Research

Project Number: 2072-21220-002-18
Project Type: Grant

Start Date: Sep 11, 2012
End Date: Sep 30, 2015

Objective:
To produce antibodies against bacterially expressed capsid proteins of four viruses from the leafroll complex to be able to develop triple-antibody sandwich (TAS) ELISA assays.

Approach:
Polyclonal antibodies produced will be tested using protocols established at University of Idaho, Moscow, and USDA-ARS, Corvallis, against the reference isolates of GLRaV-1, -2, -4, and -5 maintained in grapevine hosts at Corvallis, OR. TAS-ELISA test protocols developed will be validated on field samples collected in Nez Perce and Latah counties.

Last Modified: 10/22/2014
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