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United States Department of Agriculture

Agricultural Research Service

Research Project: Compositional Analysis and Metabolic Profiling on Diverse Maize Lines and Land Races

Location: Plant Genetics Research

Project Number: 3622-21000-038-01
Project Type: Nonfunded Cooperative Agreement

Start Date: Dec 01, 2012
End Date: Nov 30, 2014

Objective:
Maize contains an incredible amount of genetic and phenotypic diversity considering its crop history – domestication from teosinte in Mexico, adaptation and spread of landraces across the Americas prior to the Columbian exchange, and intense selection for productivity and other agronomic traits during modern plant breeding. Allelic diversity in the genes underlying seed composition has changed dramatically as a result of artificial selection during domestication and breeding, leading to the modern hybrids of today. In order to identify genes underlying kernel composition and to continue to improve kernel traits in maize, we must realize the full range of variation for these traits in maize. Many studies have been conducted to investigate small numbers of composition traits in narrow samples of maize germplasm. Here we propose to conduct a very thorough analysis of grain composition and metabolites in a set of hybrids representing the range of domesticated maize. The formal objective of this project is to investigate the natural variability of composition traits and metabolic profiles in maize grain derived from diverse inbred lines and landraces.

Approach:
The parties propose to comprehensively analyze the kernel composition and metabolite profiles in two sets of B73 hybrids with: 26 NAM inbred parents (plus Mo17) and 20 landrace inbreds. The NAM (nested association mapping) parents are inbred lines that were chosen to maximally capture genetic diversity and thus represent the scope of inbred diversity from around the world. The landrace inbreds are a set of partial inbreds (5-6 selfing generations) that were selfed directly from landrace accessions by a scientist at the University of Wisconsin. The two sets of parental lines for these hybrids have been the subject of intense phenotypic, genetic, and genomic investigation by the Maize Diversity Project, where 55 million single nucleotide polymorphisms (SNPs) were identified across this germplasm and a genome-wide domestication study identified approximately 1000 genes that experienced artificial selection during domestication and/or breeding. Replicated trials (Columbia, MO and Ithaca, NY, 3 replicates each) were grown in 2012, and plants were self-pollinated to produce seed for composition studies. Seed samples will be analyzed for kernel composition traits (e.g. proximates, amino acids, fatty acids, and selected vitamins) and submitted for metabolite profiling via GC/MS (e.g. 300 metabolites including free amino acids, sugars and organic acids). We will use established statistical methods to determine the effect of the genotype, environment, and genotype x environment on levels and interrelationships of these traits.

Last Modified: 9/10/2014
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