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United States Department of Agriculture

Agricultural Research Service

Research Project: Translational Genomics in Cucumber - Tool Development and Application for Recessive Disease Resistances

Location: Vegetable Crops Research Unit

Project Number: 5090-21000-062-24
Project Type: Trust

Start Date: Oct 01, 2012
End Date: May 30, 2015

Objective:
1) Develop a SNP-based high-resolution genetic map through genotyping by sequencing in a recombinant inbred line population; 2) Develop an integrated, high-resolution genetic-physical map with SSR and SNP markers in cucumber; 3) Conduct QTL mapping of different sources of resistance against the new downy mildew strain and powdery mildew.

Approach:
1) Development of SNP- and SSR-based high-resolution genetic map: a. Develop a mapping population with 175 RILs from Gy14 × 9930 mating b. SNP genotyping of this population by re-sequencing c. Develop a SNP- and SSR-based high-resolution linkage map with this population d. Anchoring cucumber draft genome scaffolds onto this genetic map to develop an improved chromosome-level genome assembly; 2) QTL mapping of downy mildew and powdery mildew resistances: a. Resistance sources: PI 197088, PI 330628, Gy14 (dm-1) b. Mapping populations: F2 and F2:3 from different crosses c. Phenotyping: greenhouse artificial inoculation, and field trials (both ARS and sponsor) d. Genotyping: SSRs, indels, SNP-based CAP, dCAPS e. Fine mapping of major QTL for downy mildew and powdery mildew resistances, and clarify the linkage relationships of major QTLs for the two resistance genes.

Last Modified: 12/25/2014
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