Location: Foreign Animal Disease Research
Project Number: 1940-32000-057-48
Start Date: Dec 01, 2012
End Date: Nov 30, 2014
1. Test the virulence of FMDV L sap mutant within the context of A24 which was derived from an infectious cDNA clone of FMDV field strain A 24Cruzeiro. In vivo studies will be conducted in swine utilizing different doses of A24-FMDV Lsap mutant. Clinical signs will be measured. 2. Mutant FMDV will be constructed by deleting or substituting conserved residues in the 3B region of the infectious clone of A24-FMDVL sap. Plasmids will be transcribed and resulting products analyzed. The full-length transcripts transcripts will be introduced into baby hamster kidney (BHK)-21 cells to recover infectious virus which will be characterized for in vitro activity. Mutant viruses (A24-FMDVLsap3Bmut) with an attenuated phenotype will be selected for further characterization in vivo. 3. The virulence of the mutants derived (above) will be studied in vivo in swine. Clinical signs and induction of adaptive immunity will be monitored.