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United States Department of Agriculture

Agricultural Research Service

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Research Project: Evaluation of the Population Structure of Macrophomina phaseolina and Optimization of Soil Detection and Quantification Assays

Location: Crop Improvement and Protection Research

Project Number: 5305-22000-015-13
Project Type: Reimbursable

Start Date: Feb 01, 2014
End Date: Jan 31, 2015

Objective:
The objective of the project is to evaluate the pathogenicity/virulence of isolates of Macrophomina phaseolina on strawberry, examine the population structure of the pathogen, and develop techniques for soil quantification.

Approach:
Our collaborator with th UC Cooperative Extension will collect isolates of the pathogen from field samples and evaluate them for pathogenicity on strawberry and a series of other hosts. Virulence tests will also be performed to confirm if there is any host specificity for the isolates. We will conduct SSR analysis of the populations and compare California isolates with the population structure found on other hosts in the USA, done in collaboration with an ARS scientist in Dawson, Georgia. Soil quantification will be examined by evaluating traditional soil plating assays and real time PCR methods.

Last Modified: 7/28/2014
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