Location: Plant Germplasm Introduction and Testing
Project Number: 5348-21000-025-02
Start Date: Jan 16, 2013
End Date: Jan 16, 2016
This project will focus on the three main alfalfa seed and hay production areas in the Western United States: the Walla Walla valley and Columbia Basin in Washington, the Treasure Valley in Idaho and Oregon, and Fresno County in California. Monsanto has agreed to provide us with RRA location data and their event-specific PCR primers. We will be using greenhouse seedling assays and protein based, commercially available test strips to carry out qualitative tests for RRA presence in leaf, seed and hay samples acquired along the alfalfa production pathway. Positive tests will be confirmed using PCR. Geostatistical tools will be used to understand how pollen- and seed-mediated gene flow is influenced by landscape variables and a production-level gene flow model will be developed that industry can use to guide selection of specific co-existence strategies based on location, production environment and type of pollinator used.