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United States Department of Agriculture

Agricultural Research Service

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Research Project: Improved Availability and Distribution of Sorghum Genetic Resources

Location: Plant Genetic Resources Conservation Unit

Project Number: 6607-21000-011-12
Project Type: Trust

Start Date: Feb 01, 2014
End Date: Feb 02, 2016

Objective:
1. Process 3,121 accessions, which have been regenerated and cleaned, into the U.S. sorghum and genetic stock collections. 2. Conduct seed cleaning operations on 2,487 regenerated samples of sorghum accessions presently at Griffin. 3. Conduct seed cleaning operations on 1,260 additional samples of sorghum accessions being regenerated in St. Croix and shipped to Griffin in 2014. 4. Process all regenerations cleaned in Objectives 2 and 3 into the U.S. sorghum and genetic stock collections. 5. Assist federal personnel in compiling, packaging, and shipping orders of sorghum accessions to researchers upon request.

Approach:
Two full-time employees will be hired from April through July 2014 to process into the collection 3,121 sorghum accessions that have already been cleaned. The 3,121 accessions include St. Croix regenerations of existing accessions in the sorghum collection harvested in 2012 (1,252 acc.), Schertz mutant lines (505 acc.), Schertz translocation lines (114 acc.), a RIL population (127 acc.), and sorghum accessions previously stored only at Ft. Collins, CO (1,123 acc.). Bulk seed of each accession will be stored in -18 C. Seed processing includes obtaining hundred seed weight, total seed amount, sample for germination testing, two random samples for security backup at Ft. Collins, CO and Svalbard, Norway, labeling and bar coding the seed packets, depositing the seed sample with distribution sample at 4C and bulk seed at -18C, and loading inventory and passport information to Germplasm Resources Information Network (GRIN). Unit germination personnel schedule germination testing of the sample for acceptable quality. All security backup samples are boxed and shipped to Fort Collins, CO. Processing of the 3,121 accessions should be completed in 4 months. As time permits, these personnel will assist federal personnel in filling seed distributions upon request from sorghum researchers. Two part-time personnel will be hired from July through October 2014 to conduct seed cleaning operations on 2,487 sorghum accessions that have been regenerated. These accessions include St. Croix regenerations of existing accessions in the sorghum collection harvested in 2013 (1,212 acc.), A line regenerations (130), exotic progenitor lines (57), additional Puerto Rico regenerations of sorghum accessions (909), and wild sorghum lines (179). Cleaning sorghum seed utilizes existing seed blowers and hand sorting at Unit seed cleaning work stations to remove all contaminants and trash from the seed sample. Personnel must thoroughly clean all equipment and surfaces with air hoses between samples to prevent any possible mixing of accessions. One full-time employee will be hired from April through July 2015 to process into the collection the 2,487 sorghum accessions cleaned during the previous fall following previously noted procedures. Two half-time employees will be hired from July through October 2015 to conduct seed cleaning operations on 1,260 sorghum accessions regenerated in St. Croix during 2014 and any other sorghum accessions obtained through quarantine, other acquisition, or donation. Seed cleaning operations will be conducted as noted previously. One full-time employee will be hired from August through November 2015 to process into the collection the 1,260 sorghum accessions being cleaned in 2015 as well as other sorghum accessions acquired. As time permits, these personnel will assist federal personnel in filling seed distributions, continue to split samples of 12,426 existing sorghum accessions currently in 4C, and compile sorghum images, historic data, and observational data into GRIN.

Last Modified: 9/21/2014
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