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United States Department of Agriculture

Agricultural Research Service

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Research Project: Hlb Resistance Throguh Transgenic Expression of Short Chain Fragment Variable Antibodies Against Key Liberibacter Epitopes

Location: Subtropical Insects and Horticulture Research

Project Number: 6618-21000-014-18
Project Type: Reimbursable

Start Date: Apr 01, 2012
End Date: Mar 31, 2015

Objective:
To transform citrus with genes encoding selected scFv and evaluate the transgenic citrus for resistance to huanglongbing (HLB).

Approach:
ARS USHRL Fort Pierce will use these constructs to transform citrus, identify transformed citrus, produce and propagate transformed trees, and screen them for resistance to 'Ca. Liberibacter asiaticus'. ARS U.S. Hortictural Research Laboratory, Ft. Pierce has developed efficient methods for large scale transformation, regeneration and screening of citrus for resistance to Huanglongbing (HLB). ARS Beltsville has developed an array of scFv that bind to critical epitopes on the pathogen which causes huanglongbing in citrus.

Last Modified: 8/30/2014
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