Start Date: Oct 01, 2013
End Date: Sep 30, 2018
Contemporary influenza virus isolates will be used to immunize pigs to generate swine antisera. The antiserum panels will be used in serologic assays to quantify antigenic evolution of domestic isolates and the antigenic relatedness of viruses globally to provide criteria for identifying changes of concern for swine or human health. Sequence analysis will be used to generate prediction models for cross-reactivity and subsequent cross-protection studies in swine. Likewise, genetic and antigenic analysis will be used to identify genetically variant SIV for in vivo pathogenesis and transmission studies in the swine host. Isolates will be identified based on sequence analysis, phenotyping in pigs, and/or human health events.