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United States Department of Agriculture

Agricultural Research Service

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Research Project: Genome Editing to Create Germ Cell Deficient Livestock

Location: Animal Biosciences and Biotechnology Laboratory

Project Number: 1245-31000-103-09
Project Type: Trust

Start Date: Sep 01, 2013
End Date: Aug 31, 2016

Objective:
Use site-directed genetic modification of the NANOS3 gene, via genome editing, to produce livestock (pigs) animals lacking spermatogonial stem cells (SSCs). The testes of modified males are expected to lack endogenous spermatogenesis, but provide a competent environment to support spermatogenesis initiated from transplanted genetically elite or engineered (cultured) SSCs.

Approach:
The Sponsor will provide transcription activator-like effector nucleases (TALENs) that have been validated in vitro for the ability to target and modify the NANOS3 gene. UMD-CP and ARS scientists will use these enzymes to disrupt the NANOS3 gene by site directed mutagenesis. UMD and ARS scientists will inject TALENs into zygotes which will then be transferred into recipient females Offspring will be screened for genetic alterations in the NANOS3 gene expected to result in SSC deficient males. The animals will be housed and reared in ARS facilities. ARS, UMDCP & the Cooperator will evaluate all data and publish jointly.

Last Modified: 9/10/2014
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