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United States Department of Agriculture

Agricultural Research Service

Research Project: Development of Molecular Markers Linked to Columbia Root-Knot Nematode Tuber Resistance from Solanum Tuberosum L. Clone Pa99n82-4

Location: Vegetable and Forage Crops Production Research

Project Number: 5354-21220-011-14
Project Type: Specific Cooperative Agreement

Start Date: Sep 30, 2013
End Date: Sep 30, 2015

Objective:
Columbia Root-knot nematode is a serious pest of potato in the Pacific Northwest. Resistance to root reproduction and tuber penetration have been introgressed into advanced materials. The two resistances are controlled by independent but linked dominant genes. We need to identify linked molecular markers to accelerate the selection process. The tuber resistance has no associated molecular marker at the present time. The primary objective is to develop a marker for tuber resistance.

Approach:
Within a segregating population the phenotype for tuber resistance will by determined by inoculating progeny of cross with a root+tuber resistant parent x root+tuber susceptible parent. A bulk segregant analysis using composited DNA from groups of resistant and susceptible parents will be conducted using simple sequence repeat (SSR), and single nucleotide polymorphism (SNP) markers. Furthermore, candidate genes extracted from analysis of the potato genome sequence will be screened. A bioinformatics pipeline will be developed to idnetify sequence reads that are unique to each pool. Candidate genes will be picked out sequenced potato genome to identify candidate genes and linked sequences using a Genome by Sequencing approach.

Last Modified: 7/23/2014
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