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United States Department of Agriculture

Agricultural Research Service

Research Project: Enhancing Resistance to Verticillium Wilt and Stem Nematode in Alfalfa

Location: Plant Germplasm Introduction and Testing

Project Number: 2090-21000-031-03
Project Type: Reimbursable

Start Date: Mar 01, 2014
End Date: Feb 28, 2015

Objective:
The ultimate goal of this project is to identify resistance loci associated with Verticillium wilt and stem nematode in alfalfa, to develop molecular markers closely linked to the resistance loci and to validate their usefulness in mark-assisted breeding.

Approach:
Quantitative traits such as biotic and abiotic stress resistance are most likely under the control of multiple genes and interacted with environmental factors. Identification of resistance loci that contribute to variation in complex traits, is a primary challenge in plant breeding and population genetics. In the proposed study we will use an integrated framework that allows us to merge a QTL mapping approach called ‘‘genome-wide association’’ with high-throughput genome sequencing methodologies called “genotyping by sequencing (GBS)” in order to map traits quickly, efficiently, and in a relatively inexpensive manner. This framework provides a statistical basis for analyzing marker-trait association using linkage disequilibrium. It will help to identify molecular markers associated with pest resistance in alfalfa.

Last Modified: 12/19/2014
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