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United States Department of Agriculture

Agricultural Research Service

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Research Project: Green Biopesticides: Identification

Location: Natural Products Utilization Research

Project Number: 6408-32000-001-00
Project Type: Appropriated

Start Date: Apr 18, 2014
End Date: Mar 30, 2015

Objective:
Objective 1: Discover new insecticidal active ingredients that are based on natural products and that are safe with respect to occupational exposure and with respect to the environment, including non-target effects. Objective 2: Participate in development of new products based on green biopesticides.

Approach:
A bioassay-guided investigative approach will be performed on bioactive extracts in efforts to discover bioinsecticides and repellents for use against arthopod pests. General procedures for isolation of active compounds from plants and microbes. Solvent extractions, distillations, column chromatography and thin layer chromatography will be employed. Extracts, fractions and pure compounds will be tested for insecticidal and insect repellent activity in assays being carried out routinely at collaborator's laboratories. Identification of active compounds will be done using spectroscopic methods (Ultraviolet (UV), Infrared (IR), Mass Spectroscopy (MS), Nuclear Magnetic Resonance (NMR)). Chemical modification will be performed on compound(s) that will be identified as "highly active" to improve activity or bioavailability.

Last Modified: 8/22/2014
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