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United States Department of Agriculture

Agricultural Research Service

Research Project: Early Detection of Huanglongbing (Hlb) in Nursery Trees Following Infestation with Asian Citrus Psyllid (Acp)

Location: National Clonal Germplasm Repository for Citrus & Dates

Project Number: 5310-21000-010-15
Project Type: Trust

Start Date: Jan 01, 2014
End Date: Dec 31, 2014

Objective:
1) Compare the Smart-DART™ LAMP assay for HLB and qPCR to detect the bacteria associated with HLB from roots and leaves of various scions/rootstocks under nursery conditions in a contained greenhouse following infestation with infectious ACP. 2) Validate the Smart-DART™ LAMP assay as a recognized diagnostic method for HLB 3) Transfer the technology by working with nurserymen; publishing the results in peer reviewed and industry oriented journals.

Approach:
This is a study to determine the time from when a nursery plant is exposed to an Asian citrus psyllid (ACP) to when the bacteria associated with huanglongbing (HLB) can be detected and to provide a management tool for nurserymen. Two recent developments make this trial feasible: 1) the discovery that the bacteria associated with HLB may be detected early in roots, and 2) the further development of our Smart-DART™ LAMP assay so that the sensitivity of detection is consistently two logs greater than real time quantitative PCR. Nursery trees found infested with ACP are subject to long term quarantine, suspension and cancellation of registration. The research will provide information as to how early HLB could be detected from nursery trees following infestation with infectious ACP using qPCR and the more sensitive LAMP assay. The results of this research will provide documentation of when HLB can be detected in plants once exposed to infectious ACP, and determine if there is an effect in ability to detect HLB due to different rootstocks. Nursery plants of various scions and rootstocks will be exposed to infectious ACP once, then maintained in a greenhouse for protections and following practices normally used for budwood increase blocks. At bi-monthly intervals, the roots, mature leaves, and young leaves will be tested using the Smart-DART™ LAMP assay for HLB compared to the standard protocol qPCR protocols, and monitored for symptom development.

Last Modified: 9/10/2014
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