Development of Methods for Maintenance of Lettuce-Infecting Tospoviruses and Effective Germplasm Screening
Crop Improvement and Protection Research
Project Number: 5305-22000-013-06
Start Date: Apr 01, 2013
End Date: Mar 31, 2015
1) Determine reliability of TSWV and INSV infection on susceptible lettuce when each virus is transmitted mechanically (manual inoculation) from infected spinach, pepper, or Emilia sonchifolia. 2) Compare transmission to susceptible and limited known sources of resistant lettuce, as well as wild Lactuca species in order to observe differential symptom development and determine effective scoring system. 3) (If Necessary) Repeat Objective 2 using thrips vectors for transmission of INSV and TSWV.
We will identify appropriate propagative hosts for virus stability and for inoculation of lettuce using mechanical transmission and/or thrips-based transmission of virus. Methods will be optimized for screening lettuce and other Lactuca species for symptom severity and virus concentration in greenhouse studies.