Location: National Programs
Project Number: 0500-00053-003-068-G
Project Type: Grant
Start Date: May 3, 2014
End Date: May 2, 2019
Objective:
Discern whether DON-3-G is sufficiently toxic to be included in the tolerable daily intake (TDI) for DON.
Approach:
Compare the effects of DON and DON-3-G on (1) [Ca2+]i elevation and CCK release in the cloned STC1 EEC model and (2) [Ca2+]i elevation and 5-HT secretion in cloned EC cell models. Compare the effects of DON and DON-3-G on (1)CCK release in primary murine I-cell-containing models, (2) PYY release in primary murine L-cell-containing models and (3) 5-HT secretion from intestinal whole mount preparations from mice and mink.
