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United States Department of Agriculture

Agricultural Research Service

Research Project: Identify and Develop Durum Wheat Resistant to Fusarium Head Blight Project Number: 0500-00053-003-70
Project Type: Grant

Start Date: May 05, 2014
End Date: May 04, 2015

Objective:
Project 1 - Develop Durum Wheat Resistant to Fusarium Head Blight: Develop FHB resistant durum wheat cultivars/germplasm with good agronomic and quality traits. Project 2 - Identify Sources of Resistance to Fusarium Head Blight in Durum Wheat: Screen the ICARDA durum wheat germplasm in order to identify good resistance sources of durum wheat and ultimately introgress the resistance genes into the cultivated durum wheat varieties to reduce the threat of the FHB disease.

Approach:
Project 1: A modified pedigree breeding method will be used to evaluate several segregating populations. DNA will be extracted from lines evaluated in the greenhouse and screened with robust known molecular markers at the USDA-ARS genotyping center at Fargo, ND. F5:6 lines that maintain disease severity less than 30% are advanced and evaluated for FHB resistance, agronomic, and quality traits in preliminary yield trails (PYT’s) at two locations. Selected lines from PYT’s are evaluated in advanced yield trials (AYT’s) at two locations. Selected lines from AYT’s are evaluated in elite durum advanced trial (EDA) at three locations. Lines that are selected from EDA are evaluated in the uniform regional durum nursery (URDN) at 12 locations for three years. Lines from the yield trials will be harvested for DON analysis. Project 2: 1) Screen diverse durum accessions from ICARDA for reaction to FHB in a FHB screening nursery located at Nanjing, China. 2) Re-evaluate the accessions exhibiting high levels of resistance in the preliminary screening test in the greenhouse and field in North Dakota. 3) Determine whether the new sources of resistance carry novel resistant loci by marker haplotyping for 3BS, 6B, 5A, and 3AS regions. 4) Pre-breeding: make crosses using the resistant lines and distribute them to durum wheat breeders.

Last Modified: 10/25/2014
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