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United States Department of Agriculture

Agricultural Research Service

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Research Project: Optimizing Heat Treatment in the Fields and Understanding the Molecular Mechanism Behind the Success of Thermotherapy for the Control of Citrus Hlb

Location: Subtropical Plant Pathology Research

Project Number: 6034-22000-039-49
Project Type: Reimbursable

Start Date: May 01, 2014
End Date: Apr 30, 2016

Objective:
1. To investigate the effect of heat stress on Las, associated prophages and those genes involved in the phage lytic cycle. 2. To monitor healthy and HLB-affected citrus genome-wide response to heat stress. 3. Optimize field thermotherapy to produce a standard operating procedure.

Approach:
Trees will exposed to heat. For obj. 1, leaves will be collected throughout the thermotherapy treatment. qPCR and RT-PCR will be used to determine if heat stress causes changes in Las phage. For obj. 2, RNA-seq will be used to determine how gene regulation in citrus is altered by heat stress. For obj. 3, a mathematical model will be developed to aid growers in determining the duration of heat treatment needed based on current within tent temperatures. Lastly, integrated approaches using chemicals that may be applied with heat treatment will be investigated.

Last Modified: 12/22/2014
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