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United States Department of Agriculture

Agricultural Research Service

Research Project: Development of Molecular Markers to Quantify Resistance Gene Dosage in Potato

Location: Small Grains and Potato Germplasm Research

Project Number: 2050-21000-032-17
Project Type: Trust

Start Date: Jul 01, 2013
End Date: Jun 30, 2015

Objective:
The goal of this proposal is the development of new molecular markers for the resistance genes, Ryadg, Rysto, Ry-fsto, Rx1, and Gpa2 that convey resistance to Potato virus Y, Potato virus X, and pale cyst nematode. Successful markers will determine copy number of resistance gene versions in a range of different potato populations thereby facilitating the production of families by potato breeders with a high percentage of resistant individuals.

Approach:
Cleaved amplified polymorphic sequences (CAPS) markers characterized in the literature as linked to genes, Ryadg, Rysto, Ry-fsto, Rx1, and Gpa2 will be reproduced in a small number of breeding clones and markers from resistant and susceptible lines will be cloned and sequenced. Aligned nucleotides identified following sequencing will be used to design and optimize PyroMark assays to detect resistant allele, single nucleotide polymorphisms. Usefulness of markers will be validated using progenies from segregating populations and if necessary, primers will be redesigned, optimized further, and retested on segregating populations.

Last Modified: 10/31/2014
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