Project Number: 2050-21000-032-17
Start Date: Jul 01, 2013
End Date: Jun 30, 2016
Cleaved amplified polymorphic sequences (CAPS) markers characterized in the literature as linked to genes, Ryadg, Rysto, Ry-fsto, Rx1, and Gpa2 will be reproduced in a small number of breeding clones and markers from resistant and susceptible lines will be cloned and sequenced. Aligned nucleotides identified following sequencing will be used to design and optimize PyroMark assays to detect resistant allele, single nucleotide polymorphisms. Usefulness of markers will be validated using progenies from segregating populations and if necessary, primers will be redesigned, optimized further, and retested on segregating populations.