Location: Subtropical Plant Pathology Research
Project Number: 6034-22000-039-50
Start Date: May 01, 2014
End Date: Apr 30, 2017
This project will be accomplished by: 1) identification and characterization of type D phage/prophage in Las bacteria via purification of phage particles by ultracentrifugation, rolling circle amplification, and cloning; 2) Gene expression analyses for hypothetical protein genes encoded in the prophage regions; 3) Investigation of stress effect (chemical and physical conditions) on the genes involved in the phage lytic cycle; and 4)Improvement of Las culture using Las inoculum with single or incomplete prophage/phage.