Discerning Metabolome and Transcriptome of Elite Germplasm Grown in the Field under Different Cultivation Practices
Sustainable Agricultural Systems Laboratory
Project Number: 1245-21000-142-03
Specific Cooperative Agreement
Start Date: Jul 01, 2014
End Date: Jun 30, 2019
To 1) analyze elite maize and soybean (and likely tomato) cultivars for metabolome (as a nutrient indicator) and transcriptome (as genetic marker) as affected by the growth environment; 2) compare the data from #1 for cultivars that have different prior histories, used for conventional versus non-cultivation performance; and 3) draw genetics x environment x (parameter tested) interactions.
Germplasm from ongoing ARS programs will be established in large scale research plots. Leaf and grain (where applicable) will be sampled and prepared for analysis at the level of metabolite and gene transcript profiling. Appropriate statistical packages will be employed including ANOVA and multivariate analysis to define the interactions.