Project Number: 5358-21000-044-10
Start Date: Oct 01, 2013
End Date: Sep 30, 2018
The USDA-ARS Corvallis genebank houses collections of several important tree fruit and berry crops including about 900 Rubus (raspberry, blackberry) and 500 Vaccinium (blueberry, cranberry, lingonberry) clones maintained as living plants. In recent years a number of new and potentially harmful viruses have been reported in these crops, and the incidence of these viruses in genebank collections is unknown. Important viruses in Rubus include but are not limited to Raspberry bushy dwarf (RBDV), Raspberry leaf mottle (RLMV), Raspberry leaf curl (RpLV), Black raspberry necrosis (BRNV), Rubus yellow net (RYNV), Strawberry necrotic shock (SNSV), Blackberry yellow vein (BYVaV), Blackberry chlorotic ringspot (BCRV), Blackberry Virus E (BVE), and Blackberry virus Y (BVY). Important Vaccinium viruses include Blueberry latent virus (BBLV), Blueberry latent spherical virus (BLSV), Blueberry leaf mottle (BLMoV), Blueberry mosaic (causal agent unconfirmed), Blueberry necrotic ring blotch (BNRBV), Blueberry red ringspot (BRRV), Blueberry shock (BlShV), Blueberry scorch (BlScV), Blueberry shoestring (BSSV), Peach rosette mosaic (PRMV), Tobacco ringspot (TRSV), and Tomato Ringspot (ToRSV). Some of these viruses are spreading and causing economic damage in the SE United States, in regions that were the source of some recent genebank accessions. More than a dozen of these viruses can be detected by Enzyme Linked Immunosorbent Assay (ELISA), but others can only be detected by Polymerase Chain Reaction (RT-PCR). We propose to test 1400 Rubus and Vaccinium accessions for all know viruses by ELISA and RT-PCR over two years. We also propose to examine a select group of accessions (50 per year) that are of special interest to nurseries or growers, or which have exhibited unusual symptoms not associated with know pathogens, by double stranded RNA analysis. Leaf tissue will be collected in spring or early summer at the USDA genebank.