DIFFERENTIAL GENE EXPRESSION OF MEGACHILE ROTUNDATA FEMALES PERFORMING VARIOUS NESTING BEHAVIORS
Pollinating Insects-- Biology, Management and Systematics Research
Project Number: 5428-21000-013-12
Nonfunded Cooperative Agreement
Start Date: Jun 01, 2008
End Date: May 31, 2013
The objective of this cooperative research is to raise and manipulate M. rotundata females nesting in field cages so as to capture the bees while performing specific behaviors related to different behavioral and physiological states. Genetic analysis of bees collected in the different states will be performed to determine any differential gene expression between states. This study is part of a larger sociogenomic study for exploring the molecular evolution of sociality in Hymenoptera.
Megachile rotundata adults will be reared in the ARS laboratory. The adult bees will then be released into field cages where their nesting activities will be monitored and manipulated for obtaining bees of different behavioral and physiological states. Freezer-killed adults will be shipped/transported to University of Illinois where bees will be dissected and genetic analyses will be performed.