Location: Vegetable Crops Research Unit
Project Number: 3655-21000-054-01
Start Date: Sep 23, 2009
End Date: Sep 22, 2014
Eggs will be collected from wild Hyles lineata in Arizona. Collaborators in AZ will blacklight for hawkmoths, induce the hawkmoths to lay eggs and collect the eggs from a number of different wild hawkmoths. They will ship the eggs to Madison-Wisconsin. The eggs will be maintained at high humidity and at 72F until they hatch. The larvae will be trained to feed on an artificial diet typically used to rear Manduca sexta. The larvae will go through their five instars before they become pupae. The pupae will be allowed to bury themselves in dirt until they hatch. There will be a need to go through this cycle many times before we can successfully match the eclosure of adult Hyles lineata and the flowering of the rocky mountain columbine in the greenhouse. Once these two processes are matched experiments on hakwmoth choice for floral traits will be started. Examination of how the interaction between the proboscis length of the hawkmoth and the spur length of the flower affect pollen removal and deposition by hawkmoths will be examined. The influence of floral scent, flower color, nectar production, pollen production on pollinator choice of flowers will be started together with a determination of how the pollinators select for these floral traits. The latter will be examined using a phenotypic selection experiment. In addition we will be able to pursue the pollen carryover and paternity shadow curves by hawkmoths in the greenhouse described in the parent project.