Location: Animal Parasitic Diseases
Project Number: 8042-32000-098-05
Start Date: Apr 14, 2010
End Date: Nov 30, 2014
Washington State University (WSU) will contribute to the first and third objectives. The first objective WSU will search public databases and use bioinformatic tools to examine the transcriptome of alveolar macrophages in response to PRRSV infection. This data will serve as a reference to determine what genes are expressed exclusively or predominantly in response to PRRS infection and compared to Michigan State University full transcriptome data. For the third objective, WSU will annotate specific porcine genes manually using comparative annotation procedures for retrieval of both cDNA and genomic DNA sequences of each gene identified in the second objective. WSU will then detect putative splicing forms in each of selected DE pig genes using three approaches – comparative analysis in mammals, EST evidence and PCR screening. The different sizes of products will be sequenced and the tentative splicing forms examined. Primers will be designed to target different splicing form and RT-PCR carried out to examine which form is most abundantly expressed in high or low responders to PRRSV infection.