Location: Aquatic Animal Health Research
Project Number: 6420-32000-024-01
Start Date: Jan 01, 2010
End Date: Dec 31, 2014
Antimicrobial peptides will be isolated through bioassay-guided fractionation that shows activity against important fish bacterial pathogens such as, but not limited to, Edwardsiella ictaluri, Streptococcus iniae, and Flavobacterium columnare. The source of antimicrobial peptides will be aquatic animals, such as bivalves, from which a novel antimicrobial peptides has been discovered by Qingdao Agricultural University. Bioactive crude protein extracts will be initially tested for antimicrobial activity against fish pathogens. Various fractionation techniques, including HPLC, will be used to separate, purify, and determine the active antimicrobial ingredient guided by antimicrobial bioassays.