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United States Department of Agriculture

Agricultural Research Service

Research Project: Production, Stabilization and Formulation of Microbial Agents and Their Natural Products

Location: Crop Bioprotection Research

Project Number: 5010-22410-014-00
Project Type: Appropriated

Start Date: Oct 01, 2010
End Date: Sep 30, 2015

Objective:
Objective 1: Develop liquid culture methods for producing fungal biocontrol agents by optimizing the nutritional and environmental conditions during growth for the production of an appropriate fungal propagule with optimal efficacy and storage stability. Initially, research focus will include the fungi, Metarhizium anisopliae and Mycoleptodiscus terrestris important in agricultural, urban and natural ecosystems. Objective 2: Develop novel formulation technologies for microbial biocontrol agents through the selection and application of innovative processes and ingredients that lead to improved storage stability, product delivery, field stability, and efficacy. Initially, focus will be on the fungi Isaria fumosorosea, Metarhizium anisopliae, and Mycoleptodiscus terrestris important in agricultural, urban, and natural ecosystems.

Approach:
Our approach to the development of production methods for fungal biocontrol agents will focus on the use of deep-tank, liquid fermentation techniques. Propagule form, yield, storage stability, and biocontrol efficacy are critical “fitness” factors that must be considered during medium optimization since all are required if the biocontrol agent is to become a commercial product. Assays will be performed in our laboratory to evaluate propagule “fitness” include microscopic evaluation for propagule form, measurement of the rate of biomass accumulation and propagule formation, propagule survival after drying, and propagule viability following formulation and storage. Insect or weed biocontrol assays of our fermentation products will be performed in collaboration with plant or insect pathologists. Nutritional environments that promote the rapid development (short fermentation times) of stable fungal propagules with adequate shelf-life, and consistent biocontrol efficacy will be optimized by identifying critical nutritional components in the composition of the liquid fermentation medium. In addition to nutritional parameters, environmental conditions during culture growth will be evaluated and optimized. Production and stabilization processes for promising fungal biocontrol agents will be scaled from shake-flask studies to fermentation systems as large as 100 liters depending on field trial requirements and on commercial interest. Formulation-based solutions to critical problems related to biocontrol agent stability, efficacy, and application will be addressed using a multifaceted research approach. We will evaluate the impact of culture harvest techniques, stabilization processes, and formulation ingredients on the physical characteristics, biological activity, storage stability, and field efficacy of selected biocontrol agents. Appropriate support matrices and drying processes will be evaluated for the stabilization of the microbial control agent. Support matrices to be evaluated during drying will include various diatomaceous earths, clays, and vermiculites. Processing equipment and conditions for sizing, mixing, drying, encapsulating, and granulating microbial biopesticide formulations will be tested with the ultimate goal of producing a microbial biocontrol agent in a form suitable for use against the target pest. Selective nutrients or amendments as formulations or adjuvants will be evaluated to improve biocontrol agent performance.

Last Modified: 10/22/2014
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