Start Date: Mar 01, 2011
End Date: Feb 28, 2014
1. Viruses representative of circulating genotypes will be grown to high titers in eggs. 2. Amount of antigen to be used will be compared and standardized using a monoclonal antibody that recognizes a highly conserved epitope present in all viruses. 3. The antigen will be denatured using standard techniques and injected in 4 week SPF chickens to determine immune response and protection against NDV challenge. 4. Efficacy will be determined using high pathogenicity Newcastle disease virus in 4 week old SPF chicken with measurement of protection being prevention of morbidity and mortality, reduction in number of infected birds, and a decrease in the NDV-shed from respiratory and alimentary tracts. 5. Two antigens from genotype VI and VII will be compared to a mock vaccinated and to a standard vaccine based on a lentogenic virus and three different challenge viruses will be used (4x3 a total of 12 cages).