Project Number: 5366-21000-031-04
Start Date: Aug 08, 2011
End Date: Jul 01, 2016
1) Coordinate germplasm assembly, testing protocols, and final data analysis and dissemination. Test germplasm, using a set of materials, phenotyping protocols, and experimental designs that are common to the three test locations (Davis, CA; Mt. Vernon, WA; and Corvallis, OR). Germplasm will be planted in mid-February to maximize stripe rust symptoms, reduce scald symptoms, and allow for assessment of spring, facultative, and winter germplasm. Plots will be single, 1m rows. Susceptible spreader rows, composed of mixtures of susceptible varieties, will be included. Minimum data: barley stripe rust severity at three assessment dates, heading date, other diseases. Disease samples will be sent to the USDA-ARS Pullman lab for race identification. Several categories of germplasm will be evaluated: (A) Elite germplasm--resistant and susceptible checks from the four cooperating programs (~ n = 10) ; current, new, and potential varieties (~ 3 current, 3 new, 3 proposed x 4 programs = ~ 40 entries). This ~ 50 entry PNW BSR screening trial assessed at each location using a minimum of three replicates. (B) novel--populations that may serve as resources for identifying potentially new sources of resistance, consisting of biparental and GWAS panels. Typical population sizes ~ 384. Assessed with a minimum of two replicates and a suitable design for a minimum of two years. For the 2014/2015 nursery, OSU and ARS-Aberdeen will provide one or more of the following: Biparental QTL populations segregating for BSR resistance: Full Pint x Golden Promise (n = 200 – already genotyped); Full Pint x Violetta (n = 192 – not genotyped); Full Pint x Baronesse (n = 384 – already genotyped); and 95SR316A x Lenetah (to be developed via doubled haploid (DH) n = 384). 2) Leverage T-CAP association mapping (AM) for BSR resistance in the National Small Grains Collection. This germplasm resource will have been phenotyped (2 years) and genotyped via T-CAP. Based on AM results, with assistance from this SCA and in collaboration with personnel at Aberdeen, we will introgress newly discovered QTL alleles into adapted germplasm. 3) Leverage T-CAP genotyping services provided by the USDA Regional Lab, Pullman. In collaboration with Devon See at this facility, we have developed Sequenom assays to characterize stripe rust resistance haplotypes and we will extend this to GBS in specific germplasm combinations.