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United States Department of Agriculture

Agricultural Research Service

Research Project: Finding and Deploying Genes Confering Durable Resistance to Barley Stripe Rust

Location: Small Grains and Potato Germplasm Research

Project Number: 2050-21000-031-04
Project Type: Specific Cooperative Agreement

Start Date: Aug 08, 2011
End Date: Jul 01, 2016

Objective:
Develop and deploy effective resistance against barley stripe rust (BSR)(incited by Puccinia striiformis f. sp. hordei) to protect U.S. barley production via the following activities: 1) Conduct a coordinated germplasm evaluation effort involving USDA-ARS Aberdeen and Pullman; Oregon State University, Corvallis; and University of California, Davis; 2) Discover new resistance QTL via analysis of data from germplasm evaluation and leverage T-CAP association mapping data results for BSR; 3) Introgress current barley stripe rust resistance QTL into elite breeding populations via marker assisted selection (MAS). Climate change, with increasingly volatile swings in climate, can lead to diseases appearing in heretofore “safe” havens. Thus, while stripe rust of barley has been most prevalent in the high rainfall areas of the Pacific Northwest, it could emerge as a threat elsewhere in the US with a simple shift in weather patterns and cropping systems. Thanks to systematic mapping and introgression efforts, a catalog of mapped resistance genes and QTLs is available as is elite, adapted germplasm. The price of healthy crops, however, is eternal effort.

Approach:
1) Coordinate germplasm assembly, testing protocols, and final data analysis and dissemination. Test germplasm, using a set of materials, phenotyping protocols, and experimental designs that are common to the three test locations (Davis, CA; Mt. Vernon, WA; and Corvallis, OR). Germplasm will be planted in mid-February to maximize stripe rust symptoms, reduce scald symptoms, and allow for assessment of spring, facultative, and winter germplasm. Plots will be single, 1m rows. Susceptible spreader rows, composed of mixtures of susceptible varieties, will be included. Minimum data: barley stripe rust severity at three assessment dates, heading date, other diseases. Disease samples will be sent to the USDA-ARS Pullman lab for race identification. Several categories of germplasm will be evaluated: (A) Elite germplasm--resistant and susceptible checks from the four cooperating programs (~ n = 10) ; current, new, and potential varieties (~ 3 current, 3 new, 3 proposed x 4 programs = ~ 40 entries). This ~ 50 entry PNW BSR screening trial assessed at each location using a minimum of three replicates. (B) novel--populations that may serve as resources for identifying potentially new sources of resistance, consisting of biparental and GWAS panels. Typical population sizes ~ 384. Assessed with a minimum of two replicates and a suitable design for a minimum of two years. For the 2014/2015 nursery, OSU and ARS-Aberdeen will provide one or more of the following: Biparental QTL populations segregating for BSR resistance: Full Pint x Golden Promise (n = 200 – already genotyped); Full Pint x Violetta (n = 192 – not genotyped); Full Pint x Baronesse (n = 384 – already genotyped); and 95SR316A x Lenetah (to be developed via doubled haploid (DH) n = 384). 2) Leverage T-CAP association mapping (AM) for BSR resistance in the National Small Grains Collection. This germplasm resource will have been phenotyped (2 years) and genotyped via T-CAP. Based on AM results, with assistance from this SCA and in collaboration with personnel at Aberdeen, we will introgress newly discovered QTL alleles into adapted germplasm. 3) Leverage T-CAP genotyping services provided by the USDA Regional Lab, Pullman. In collaboration with Devon See at this facility, we have developed Sequenom assays to characterize stripe rust resistance haplotypes and we will extend this to GBS in specific germplasm combinations.

Last Modified: 11/27/2014
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