Location: Grain, Forage & Bioenergy Research
Project Number: 5440-21220-032-04
Start Date: Feb 01, 2011
End Date: Jan 31, 2016
To increase carbon flux through the phenylpropanoid pathway, we will introduce seven constitutive expression cassettes under control of the maize ubiquitin promoter. We will constitutively express the genes encoding 7 enzymes in the sorghum monolignol pathway: cinnamate 4-hydroxylase, 4-coumarate-CoA ligase, hydroxycinnamoyl CoA:shikimate/quinate transferase, caffeoyl CoA O-methyltransferase, ferulate 5-hydroxylase, caffeic acid O-methyltransferase and cinnamyl alcohol dehydrogenase in RTx430 sorghum. Agrobacterium-mediated transformation will be used coupled to npt II selectable marker. For each transgenic cassette a minimum of 10 independent events will be generated.