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United States Department of Agriculture

Agricultural Research Service

Research Project: Sequencing the Cranberry Genome

Location: Genetic Improvement of Fruits and Vegetables

Project Number: 1245-21000-276-04
Project Type: Nonfunded Cooperative Agreement

Start Date: Sep 29, 2011
End Date: Apr 30, 2014

Objective:
To complete sequencing and assembly of the cranberry genome via bioinformatics, making the cranberry genome publically available and for joint ARS and Cooperator's research. Ultimately, assembly will inter alia assist in mapping, accession identification, marker-assisted selection, and gene discovery.

Approach:
1. Generate a draft genome from the SOLiD sequence data already acquired. 2. Create a new genomic library from the same accession, using 500 and/or 250 kb inserts, for Illumina/Solexa sequencing to improve the draft assembly. 3. Assemble the genome using bioinformatics. 4. Identify gene markers in the cranberry genome through Simple Sequence Repeats (SSRs) in the genome and isolate genes of interest.

Last Modified: 8/22/2014
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