Genomic and Functional Genomic Analysis of Fungal Plant Pathogens
Floral and Nursery Plants Research Unit
Project Number: 1230-22000-049-02
Specific Cooperative Agreement
Start Date: May 01, 2011
End Date: Sep 30, 2014
The objectives of this agreement are to analyze Rhizoctonia solani and related pathogens using: (1) high-throughput genome assembly, gene identification, annotation and comparative genomics, and (2) transcriptome sequence analyses to aide in functional genomics and in genome annotations. Sequence information will enable accurate identification of Rhizoctonia AG groups which is critical in identifying host-pathogen interactions leading to disease control strategies. Genome sequencing will also lead to improvements in resistance breeding, development of new fungicides based on alternate metabolic pathways, and in effective monitoring of fungicide resistance in the pathogen population. The Cooperator has the necessary bioinformatic expertise that we do not for the analyses including development and annotation of the full genome and transcriptome sequences of plant pathogens.
ARS will develop genome and transcriptome sequences of R. solani and related pathogens using high throughput sequencing approaches. This information will be used by both parties to jointly develop a greater understanding of the biology, ecology and pathology of this organism through computational and other bioinformatic and wet-lab experimentations. The Cooperator will generate bioinformatic tools and databases which will facilitate molecular investigations of the pathogen.