Project Number: 6402-21310-004-12
Start Date: Jul 26, 2012
End Date: Jul 25, 2016
Genomic data will be generated in two distinct areas. 1) Reduced representative DNA libraries will be sequenced using a high throughput DNA sequencer. As peanut is a tetraploid with a large genome size it is still too expensive to do whole genome resequencing. This method selectively reduces the areas sequenced but provides useful coverage over many gene regions and allows for the discovery of single nucleotide polymorphisms (SNP) which are useful for genetic mapping. 2) Gene expression data will be generated using a technique called RNA-Seq. Peanut tissue with spatial and temporal differences will be examined.