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United States Department of Agriculture

Agricultural Research Service

Research Project: Antibiotics and Their Biosynthetic Intermediates: Inter-Cellular Chemical Messengers Mediating Genome-Wide Transcriptional Effects in Soil

Location: Horticultural Crops Research

Project Number: 2072-12220-004-14
Project Type: Specific Cooperative Agreement

Start Date: Sep 01, 2011
End Date: Jan 31, 2015

Objective:
1. Determine the influence of phloroglucinol and other metabolites of Pf-5 on the transcriptome and antibiotic-production profiles of the bacterium 2. Evaluate the role of phloroglucinol in cell-to-cell communication between strains of P. fluorescens.

Approach:
Global patterns of gene expression by P. fluorescens will be assessed using RNA-Seq, and statistical methods employed to identify genes whose expression is altered by exogenous sources of phloroglucinol or other metabolites. Metabolic profiles will be generated by HPLC analysis of extracts from cultures of P. fluorescens.

Last Modified: 11/25/2014
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