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United States Department of Agriculture

Agricultural Research Service

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Research Project: INTERACTIONS BETWEEN AMBROSIA BEETLES AND THEIR FUNGAL SYMBIONTS

Location: Biological Integrated Pest Management Unit

Project Number: 8062-22000-019-13
Project Type: Specific Cooperative Agreement

Start Date: Sep 14, 2011
End Date: Sep 13, 2014

Objective:
The goal of this project is to isolate and characterize strains of fungi (Ambrosiella spp.) associated with ambrosia beetles. Objectives include 1) Identify and assess genetic diversity among strains isolated from Xylosandrus crassiusculus and X. germanus and 2) Determine impact of fungal diversity on beetle development within various hosts and substrates.

Approach:
Ambrosia beetles (Xylosandrus spp. and others) are pests of a wide array of woody plants used in the nursery industry. They depend, for their survival, on their own cultivation of a fungus (Ambrosiella spp. and others). Fungal strains isolated from Xylosandrus spp. will be identified and assessed through sequencing of 18S, 28S and ITS regions of ribosomal DNA from X. crassiusculus and X. germanus. Brood development and symbiont growth will be evaluated for each fungal genotype on representative hosts or diet substrates. Fecundity of beetles within galleries will be evaluated.

Last Modified: 10/24/2014
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