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United States Department of Agriculture

Agricultural Research Service

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Research Project: Durable Rust Resistance in Wheat - Phase II

Location: Cereal Disease Laboratory

Project Number: 5062-21220-021-11
Project Type: Reimbursable

Start Date: Mar 01, 2011
End Date: Feb 29, 2016

Objective:
Identify molecular markers linked to stem rust resistance genes, identify new sources of resistance to stem rust, and characterize the molecular genetics of stem rust resistance in order to deploy resistant varieties of wheat to Ug99 stem rust in the United States and worldwide.

Approach:
Seedling Phenotyping of Mapping Populations. Stem rust assays of seedling plants from mapping populations can be an efficient way to provide phenotypic data for use in the identification of markers linked to stem rust resistance. The Cereal Disease Laboratory has provided wheat geneticists with phenotypic data for mapping populations segregating for stem rust resistance genes including Sr13, Sr22, Sr35, SrTmp, and a number of populations being mapped by CIMMYT and other collaborators associated with the DRRW projects. We request support for further phenotyping of mapping populations segregating for new and unknown resistance genes. Seedling Screening of Breeding Lines and Diverse Germplasm. Screening breeding lines at the seedling stage with diverse races of stem rust including Ug99 lineage and other important virulence combinations at the Cereal Disease Laboratory has helped to postulate Ug99-effective genes in various gene pools and breeding germplasm, including confirmation of APR resistance. This work has provided breeders in CIMMYT, ICARDA, Ethiopia, Kenya, and United States with information on the presence or absence of particular resistance genes and effective gene combinations. The Cereal Disease Laboratory can continue to provide seedling screening of breeding lines and germplasm with Ug99, variants of Ug99, and other virulent races in order to provide breeders with the necessary information to develop/improve stem rust resistance. Molecular Genetics of Rust Resistance near the Sr9 locus. Recent data indicate the presence of a Ug99 resistance locus linked or allelic with the Sr9 locus (resistance genes temporarily designated as SrWeb and SrGabo56). Several stem rust alleles are present at the Sr9 locus which is also known to be allelic with stripe rust resistance genes Yr5 and Yr7. USDA-ARS scientists at Pullman, WA, are conducting studies in order to clone Yr5. The availability of markers and sequence information at the Yr5 locus will enable the development of markers potentially tightly linked with Sr9 and SrGabo56 in available mapping populations. In order to test for the allelism of SrGabo56 and Sr9, populations for testing allelism have been derived. Screening of these populations with both stem rust and molecular markers can determine whether or not SrGabo56 is an allele of Sr9. The unique phenotypic diversity at the Sr9 locus makes this an appealing candidate for cloning. Understanding the variation responsible for five Sr9 alleles, Yr5, Yr7, and SrGabo56 will uniquely enhance our understanding of the molecular mechanisms of resistance and race-specificity and could provide base knowledge for long-term projects including the development of new resistance alleles and for the deployment of durable resistance. The marker information available from the mapping of Sr9a, SrWeb, SrGabo56, and Yr5, the sequence information available from the Yr5 mapping project, and the plant populations currently available make the molecular dissection of resistance at this locus feasible.

Last Modified: 12/18/2014
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