Location: Horticultural Crops Research
Project Number: 5358-21000-047-02
Start Date: Jan 20, 2014
End Date: Sep 30, 2015
Industry cooperators who participated in the preliminary work will be contacted for vineyard access, so this work can begin immediately (at notification of funding). For red cultivars, a minimum of twelve vines per vineyard (six comparison pairs) will be selected based on visual symptoms, consisting of six control-healthy and six of suspected infection, which will be tested for virus infection. For white fruited cultivars, identification of infected and healthy vines will be based on virus testing, since there is no reddening of the leaves. For fruit sampling three pairs of infected and healthy vines, as determined from the virus testing, will be used for analysis. Sampling will occur for two growing seasons, as replication prevents a single irregular growing season from skewing our findings. Grape clusters at commercial harvest will be obtained from healthy and GRLBV infected vines. These grapes will be tested for a suite of viruses previously identified (PCR and/or ELISA tests as appropriate for each virus; Krenz et al., 2012). A fruit subsample will be taken for phytochemical analysis by recognized protocols.