Location: Appalachian Fruit Research Laboratory
Project Number: 8080-21000-023-09
Start Date: Apr 15, 2012
End Date: Apr 14, 2017
This work will be accomplished using a new strategy pioneered by the PDs that enables rapid identification of a single or small number of candidate genes responsible for single locus traits in peach. Briefly, it involves sequencing pooled genomes from segregating populations using next generation sequencing technologies. The resulting data will be mined for polymorphisms, and allele frequencies from individual genome pools will be calculated. Finally, the frequencies are graphed by chromosome position to simultaneously reveal the physical location of the trait and the causative polymorphism. Identified candidate genes will be confirmed via molecular markers designed from the genome sequences and functional tested by transformation into plum.